Callus Induction

Callus induction refers to the horticultural technique of stimulating undifferentiated plant tissue (callus) formation in cannabis cuttings or explants, typically through application of plant growth regulators like auxins and cytokinins. This process is foundational to micropropagation and tissue culture workflows, allowing breeders and researchers to rapidly multiply elite genetics while maintaining genetic fidelity. Callus tissue can be induced from leaf, stem, or root segments under controlled laboratory conditions, and subsequently differentiated into shoots or roots depending on hormone ratios. Understanding callus formation is critical for cannabis breeding programs seeking to preserve rare or unstable phenotypes, scale superior clones, and establish genetic repositories. The technique bridges traditional cloning with modern cellular agriculture approaches within cannabis horticult

Breeders employ callus induction to bypass seed-based propagation bottlenecks, rapidly multiply F1 hybrids or unique cultivars without somatic variation, and create backup genetic material. This is particularly valuable for preserving heterozygous or chimeric phenotypes that cannot reliably breed true.